Flowcytometry Laboratory Unit
NUHS Flow Cytometry Laboratory provides supports to NUS researchers and external groups from other research institutes. The facility is equipped with two analyzers (CyAnTM ADP Analyzer - Beckman Coulter; BD LSR Fortessa Analyzer- Beckton Dickinson) and one sorter (BD FACSVantage SETM - Beckton Dickinson), as well as a magnetic-bead separator (SuperMACSTM II Separator - Miltenyi Biotec).
Common procedures performed in the unit include DNA cell cycle analysis, apoptosis and necrosis studies, multi-parameter immunophenotypic analysis, calcium flux analysis, as well as sterile fluorescence and magnetic-based sorting.
The staffs provide training to users new to flow cytometry. In addition, they are available for consultation on experiment design, data analysis, and data interpretation.
- CyAnTM ADP
Our CyAnTM ADP is equipped with three solid state lasers (407nm, 488nm, and 633nm). It delivers high speed and multicolor analysis measuring up to nine fluorescence parameters, in addition to Forward Scatter (FSC) and Side Scatter (SSC).
- BD LSR Fortessa Flow Cytometry Analyser
Our BD LSR Fortessa is equipped with four solid lasers (405nm, 488nm, 561m and 640nm). It can detect 16 colors simultaneously in addition to Forward Scatter (FSC) and Side Scatter (SSC). It can also perform 5-color analysis without compensation.
- BD FACSVantage SE
Our BD FACSVantage SE is equipped with one Coherent Innova 90 laser (488nm). It measures three fluorescence parameters, as well as FCS and SSC, and is able to sort up to 5,000 cells/second.
- SuperMACSTM II Separator
SuperMACSTM II Separator is a magnetic-based sorter, providing alternative to the more common fluorescence-based sorting. SuperMACSTM II Separator allows for high speed sorting with more than 1011 cells separated in one step.
||(Click HERE to download Usage Record Form
| ||Application||Users||Rates Per Hour|| |
|User Operated||Staff Operated|
||NUS & NUHS Users
|NUS & NUHS Users
Training will be provided free-of-charge until the users are competent in operating the instruments themselves within a reasonable period of time as determined by our staff.
The use of our facility is by appointment only (at least 1 week in advance).
Users must give at least 24 hours notice to cancel an appointment, failing of which charge as per booking will be applied.
Billing begins from the time the appointment is scheduled to start, please be punctual. If users are late and run into next person's appointment, they must STOP acquiring samples and finish at a later time, subject to availability.
The cell sorting flow cytometers will not run samples that are potentially infectious to human.
Prof Hanry Yu
Ms Wang Xiaoning
Tel: 6516 7990
Ms Toh Chin Min
Tel: 6516 7990
Click HERE to download the Application Form.
9am to 12noon
1:30pm to 6pm
9am to 12noon
1pm to 5:30pm
Yong Loo Lin School of Medicine
National University of Singapore
#B1-03, Tahir Foundation Building (MD1), 12 Science Drive 2.